The amylases of pseudomonas saccharophila.

Some aspects of the induced biosynthesis of alpha-amylase of Pseudomonas saccharophila.

In a paper on the facultative autotroph, Pseudomonas saccharophila, Doudoroff (1940) noted that this organism forms an extracellular amylase when grown with starch as a source of carbon. Later studies showed that this enzyme could be formed also in resting cellular suspensions simply by the addition of starch or maltose (Klein and Doudoroff, unpublished experiments). In this respect, P. sacchar...

Sulfur incorporation into the alpha-amylase of Pseudomonas saccharophila.

Studies by Rotman and Spiegelman (1954) and Hogness et al. (1955) on the 3-galactosidase of Escherichia coli have revealed that virtually all of the carbon and sulfur of that enzyme is derived from exogenous sources when cells are induced during growth. These investigations have thus ruled out the direct participation of a specific preformed precursor in the formation of fl-galactosidase in gro...

On the sources of carbon for the induced biosynthesis of alpha-amylase in Pseudomonas saccharophila.

Recent experiments by Rotman and Spiegelman (1954) and by Hogness et al. (1955) have indicated that virtually all of the carbon and sulfur for the induced biosynthesis of /3-galactosidase comes from the medium rather than from any pre-existing compounds within the cell, using exponentially growing cells of Escherichia coli. These experiments also proved that the proteins of E. coli do not break...

Phosphorolysis of sucrose by Pseudomonas putrefactions.

The phosphorolytic breakdown of sucrose was discovered several years ago by three groups of investigators working independently with two unrelated genera of bacteria. This mechanism was first described in Leuconostoc mesenteroides by Kagan, Latker, and Zfasman (1942). Without knowledge of this discovery Doudoroff, Kaplan, and Hassid (1942) found the same reaction in Pseudomonas saccharophila. I...

The metabolism of D-galactose in Pseudomonas saccharophila.